Ozone Production by Dielectric Barrier Discharge Plasma for Microbial Inactvation in Rice

Muhammad Nur1*, Aribat Solichin1, Endang Kusdiayantini2, Tri A. Winarni4, Susilo5, Dian Arif Rahman1, Resti Maryam1, Sosiowati Teke1, Wuryanti3 and Harjum Muharam6
1Department of Physics, Faculty of Science and Mathematics, Diponegoro University, Semarang, Indonesia
2Department of Biology, Faculty of Science and Mathematics, Diponegoro University, Semarang, Indonesia
3Department of Chemistry, Faculty of Science and Mathematics, Diponegoro University, Semarang, Indonesia
4Department of Food Technology, Faculty of Fisheries and Marine Science, Diponegoro University, Semarang, Indonesia
5Department of Mechanical Engineering, Faculty of Engineering, Diponegoro University, Semarang, Indonesia
6Department of Management, Faculty of Economics and Business, Diponegoro University, Semarang, Indonesia
(*Tel : +62-24-7474754; *E-mail: m.nur@undip.ac.id)

Abstract- Research on ozone production by using silent plasma or Dielectric Barrier Discharge Plasma (DBDP) and implemented for microbial inactivation in rice has been done. The plasma reactor for ozone generating were reactor with multi-point-plane (MP-P) reactor and spiral-cylender (S-C) reactor configurations. The high voltage AC has been used with voltage up to 40 kV and maximum frequency 23 kHz. For implementation in rice, this research used a Randomized Block Design (RBD) with 5 treatments and 4 replications in each treatment. Exposure time was varied such as: 0, 30, 60, 90, and 120 minutes. Total bacteria and molds (fungi) has been detected by using Total Plate Count (TPC) methods with nutrient broth as media for bacteria and, we found that MP-P Reactor needed less voltage comparing with S-C Reactor for the same cocentration ozone. Ozone generated by DBDP can reduce the population of bacteria and fungi in rice. The exposure for 90 minutes can eliminate 58.41% population of bacteria, and 26.23% population of fungi. Through analysis of variance known that ozone treatment is significantly affect the total bacteria at significance level of 5% and 1% (P 0.05). Subtances of water, fat, and protein in rice was not change before and after ozone treatment.

Keywords:Plasma, ozone, rice, bacteria, fungi
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